Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 12;47(16):8807–8820. doi: 10.1093/nar/gkz600

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — ATPase-deficient New1-EQ₂ inhibits cell growth and stably co-sediments with polysomal fractions. (A) Expression of New1-EQ₂-TAP, but not wild-type New1-TAP causes growth inhibition. The strains were grown in SC-ura medium at 30°C and expression of New1-TAP or New1-EQ₂-TAP was induced by addition of β-estradiol to final concentration of 1 μM as indicated by the green arrow. OD₆₀₀ measurements are presented as geometric means of three independent transformants, and standard error of the mean is indicated with shading. (B) Polysome profile and immunoblot analyses of yeast strains expressing New1-TAP or New1-EQ₂-TAP. The cells were grown at 30°C and at OD₆₀₀ ≈ 0.3 protein expression was induced by 1 μM β-estradiol. The cells were collected after 3 h, clarified lysates resolved on sucrose gradients (either in the absence of nucleotides or supplemented by 1 mM ATP/AMP-PNP), and TAP-tag detected by slot-blotting using rabbit Peroxidase-anti-peroxidase. Full-size Western blots of wild-type and EQ₂ New-TAP are presented on Supplementary Figure S5C.