Table 1.
Strains and plasmids used in this study
| Name | Description | Reference |
|---|---|---|
| Strains | ||
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | (75) |
| BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | (75) |
| BY4709 | MATα ura3Δ0 | (75) |
| MJY944 | MATa his3Δ1 leu2Δ0 ura3Δ0 new1Δ::HIS3MX6 | This study |
| MJY945 | MATα his3Δ1 leu2Δ0 ura3Δ0 new1Δ::HIS3MX6 | This study |
| MJY1091 | MATa his3Δ1 leu2Δ0 ura3Δ0 new1Δ::kanMX6 | This study |
| MJY1171 | MATα ura3Δ0 | This study |
| MJY1173 | MATα ura3Δ0 new1Δ::kanMX6 | This study |
| MJY951 | MATα his3 leu2Δ0 ura3Δ0 new1Δ::HIS3MX6 PYEF3::kanMX6-PMET25 | This study |
| PY116 | ura3-52 trp1Δ his3-11,15 pep4-4 prb1-1122 prc1-407 leu2-3,112 nuc1::LEU2 | (33) |
| VHY61 | MATa leu2Δ0 met15Δ0 ura3Δ0 his3Δ1::FRP880 | This study |
| VKY8 | MATα his3 leu2Δ0 ura3Δ0 PYEF3::kanMX6-PMET25 | (18) |
| VKY9 | MATα his3 leu2Δ0 ura3Δ0 | (18) |
| VKY20 | MATa his3 leu2Δ0 lys2Δ0 ura3Δ0 yef3Δ::kanMX6 /pRS316-YEF3 | (18) |
| YSC1178-202233783 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 NEW1::TAP-HIS3MX6 | (76) |
| Plasmids | ||
| FRP880 | PACT1(-1-520)-LexA-ER-haB112-TCYC1 | (31) |
| FRP1642 | PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1 | (31) |
| pDB722 | PPGK1-Renilla-CAACcassette-Firefly-TCYC2, URA3, 2μ ori | (66) |
| pDB723 | PPGK1-Renilla-UAACcassette-Firefly-TCYC2, URA3, 2μ ori | (66) |
| pBS1539 | CBP-ProtA Kl-URA3 AmpR | (77) |
| pFA6a-kanMX6 | kanMX6 AmpR | (30) |
| pFA6a-HIS3MX6 | HIS3MX6 AmpR | (30) |
| pRS315 | YCp CEN6/ARS4 LEU2 AmpR | (78) |
| pRS316 | YCp CEN6/ARS4 URA3 AmpR | (78) |
| pRS425 | YEp 2μ ori LEU2 AmpR | (79) |
| pRS426 | YEp 2μ ori URA3 AmpR | (79) |
| pRS315-NEW1 | NEW1 in pRS315 | This study |
| pRS315-YEF3 | YEF3 in pRS315 | (18) |
| pRS316-NEW1 | NEW1 in pRS316 | This study |
| pRS316-YEF3 | YEF3 in pRS316 | (18) |
| pRS425-NEW1 | NEW1 in pRS425 | This study |
| pRS426-YEF3 | YEF3 in pRS426 | This study |
| pRS426-NEW1 | NEW1 in pRS426 | This study |
| VHp123 | 6His-NEW1ΔQ/N-EQ2 (ΔP2-P140 E713Q E1058Q, codon optimised to E. coli expression) in pET19b | This study |
| VHp257 | insul-(lexA-box)4-PminCYC1-NEW1E713Q-TAP-Tsynth8 in pRS316 | This study |
| VHp258 | insul-(lexA-box)4-PminCYC1-NEW1E1058Q-TAP-Tsynth8 in pRS316 | This study |
| VHp262 | insul-(lexA-box)4-PminCYC1-NEW1-TAP-Tsynth8 in pRS316 | This study |
| VHp265 | insul-(lexA-box)4-PminCYC1-NEW1-EQ2-TAP-Tsynth8 in pRS316 | This study |
| VHp327 | PTDH3-NEW1 in pRS425 | This study |
| VHp603 | PPGK1-Renilla-YDR099W(TAA)cassette-Firefly-TCYC2, URA3, 2μ ori | This study |
| VHp605 | PPGK1-Renilla-YNL247W(TAG)cassette-Firefly-TCYC2, URA3, 2μ ori | This study |
| VHp606 | PPGK1-Renilla-YDR099W(CAA)cassette-Firefly-TCYC2, URA3, 2μ ori | This study |
| VHp607 | PPGK1-Renilla-YNL247W(CAG)cassette-Firefly-TCYC2, URA3, 2μ ori | This study |