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. 2019 Nov 14;47(22):11850–11860. doi: 10.1093/nar/gkz1039

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Distance measurements obtained by DEER for isolated MR11, its binary mixture with 40S subunits and its mixture with the ternary complex of 40S subunits with 19-mer mRNA GACUUCAACAAACACAACU and P site tRNA^Asp. (Left) Background corrected four-pulse DEER traces (intensity is normalized). Lines with noise correspond to the experimental data. Solid lines show best fits obtained using DeerAnalysis2016. (Right) Obtained distance distributions. Note that distance distribution in the presence of 40S subunits is quite broad and probably contains two unresolved peaks from the unfolded and the compact MR11 forms.