Figure 9.

Distance measurements by DEER in binary mixture of MR11 with cross-linked 40S subunits, with 40S subunits subjected to the same treatments as cross-linked without any additional components and in the presence of unmodified nonaribonucleotide AAUAAAUAU or the 20-fold excess of the unmodified 11-mer UGUGUUCUAAA. (Left) Background corrected four-pulse DEER traces (intensity is normalized). Lines with noise correspond to the experimental data. Solid lines show best fits obtained using DeerAnalysis2016. (Right) The obtained distance distributions. Differences in the distance distribution are the result of experimental noise (see SI, Supplementary Figures S8 and S9).