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. 2019 Nov 15;48(1):359–372. doi: 10.1093/nar/gkz1065

Figure 5.

Figure 5.

Genetic interaction between RbfA and IF3. (A) Growth of E. coli strains in rich LB media at 37°C (OD600nm). WT, parental; IF3-Y75N, infC362; –, empty vector; and RbfA+, + p15BHA (RbfA overexpression). Mean and s.d., n = 4 biological replicates. (B) Growth of strains in (A) in minimal MOPS media (pH 7.2) supplemented with 0.4% glucose at 37°C; n = 2. (C) Growth of strains as in (A) on LB agar media plates at 37°C and 18°C; n = 3. (D) WT and IF3-Y75N cells harboring empty vector or p15BHA were transformed with pD421-rsgA for RsgA overexpression. Plates were incubated overnight at 37°C. Single colonies were subsequently streaked on a fresh plate. No transformation was observed for the IF3-Y75N/RbfA+/RsgA+ strain, n = 2. (E) Polysome profiles of strains infC362 (blue) and infC362/p15BHA (red), grown under similar conditions as (A) and collected at OD600nm ∼0.2. Experiment was performed in duplicate. (F) Serial dilutions of the indicated strains were spotted on LB-agar containing sublethal concentrations of kanamycin (2 μg/ml) or neomycin (2 μg/ml). Ampicillin (100 μg/ml) was added to all plates to maintain the plasmid; n = 2.