Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 15;47(16):8485–8501. doi: 10.1093/nar/gkz594

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 7. — Role of Zscan4-associated GABF complex in MT2/MERVL activation. (A) Western blot analysis of Flag-Zscan4c/Brg1/Brd9 co-immunoprecipitation in Flag-Zscan4c overexpressed ESCs or in control vector overexpressed ESCs. IP, immunoprecipitation. IP was done with anti-Flag antibody. 1.25% input was loaded as control. (B–D) Western blot analysis of Brg1/Bd9 co-immunoprecipitation with HA-zinc finger domains (B), HA-SCAN domain (C), and HA-internal domain (D) of Zscan4c. IP was done with anti-HA antibody and separated by SDS/PAGE. 1.25% input was loaded as control. (E) Enrichment heatmap of Brd9 and Brg1 around the binding regions of Zscan4c. The ChIP-seq signal was calculated as the ratio of normalized reads relative to input. (F) ChIP-qPCR analysis of Brg1 and Brd9 enrichment on MT2 in control ESCs (Control OE) and Zscan4c-overexpressed (Zscan4c OE) ESCs. Biological triplicate data were presented as mean ± s.e.m. (G) Relative Luciferase activity of vector containing MT2 in control ESCs (Control OE) and Zscan4c-overexpressed (Zscan4c OE) ESCs treated with inhibitor of Brd9 (BI7273). DMSO treated samples were included as control. Biological triplicate data were presented as mean ± s.e.m. (H) Schematic plot of Zscan4c function in activating MT2/MERVL and 2-cell/4-cell cleavage genes. In the absence of Zscan4c, MT2 enhancer activity is not activated and MT2 nearby 2-cell/4-cell genes are silenced. In the presence of Zscan4c, Zscan4c binds to MT2, interacts with GBAF complex member Brg1 and Brd9, and augments H3K4me1, H3K27ac, and H3K14ac deposition on MT2/MERVL, thus activates MT2 which drives the expression of MERVL and enhances the expression of 2-cell/4-cell genes.