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. 2019 Nov 13;47(22):11826–11838. doi: 10.1093/nar/gkz1049

Figure 6.

Figure 6.

Effects of KIN and CPD mutations on Candida Trl1 activity in vivo. S. cerevisiae trl1Δ p[CEN LEU2 CalTRL1] strains with CalTRL1 alleles as specified on the left were grown in YPD liquid culture at 30°C and adjusted to the same A600. Serial 10-fold dilutions were spotted to YPD agar and incubated at the 30°C and 37°C. Photographs of the plates are shown. Lethal CalTRL1-Ala alleles that failed to complement trl1Δ are indicated at the bottom.