Table 1.

CAI Values for CzNLR–CDSs Compared against the Chromochloris zofingiensis Nuclear Genome Codon Usage Table

Gene IDa	CAI (average)	e-CAIb (α = 0.05)	Statistical Tests (α = 0.05)
			K–Sc	χ 2 (confidence level)d (%)
UNPLg00136	0.606	0.664	0.032	100
UNPLg00133/134/135	0.621	0.683	0.028	100
UNPLg00039	0.614	0.676	0.034	100
UNPLg00138	0.616	0.678	0.032	100
UNPLg00127	0.614	0.674	0.030	100
UNPLg00129/130/131	0.611	0.675	0.021	100

Note.—For each CzNLR–CDS the CAI value, e-CAI value (determined by randomly generating 500 sequences with the same GC content and AA composition of CzNLR–CDSs) and statistical parameters (chi-square, χ² goodness-of-fit test and Kolmogorov–Smirnov, K–S test) are reported.

^aWith respect to the new NLR gene annotation performed in this study (see fig. 3C).

^be-CAI is a threshold value for discerning whether the differences in the CAI value are statistically significant. The e-CAI value was calculated at a 95% level of confidence using the Markov method.

^cK–S test confirmed whether the CAI of the randomly generated sequences follow a normal distribution. K–S test values for each calculated e-CAI is always lower than of critical value (0.061).

^dA χ² test is conducted to compare the goodness-of-fit between the AA frequencies or GC content of each CzNLR–CDS and their mean values.