Figure 5.

Comparison of transcriptional output of mitochondrial intronless genes in P2 and P3 phenotypes of rps10 and wild-type. Transcriptional output is defined as mtRNA-seq TPM (reads per kilobase per million reads mapping to CDS). (A) Relative mRNA level defined as mtRNA-seq in rps10 compared with WT. Log₂ of the ratio is shown. Transcripts are divided into groups encoding: ribosomal proteins, proteins of individual OXPHOS complexes, proteins involved in cytochrome c biogenesis, maturase (MatR) and transport membrane protein (TatC). (B) Level of selected mitochondrial transcripts in wild-type and rps10 analyzed by Northern hybridization. Total mitochondrial RNA was electrophoresed in denaturing conditions on 1% agarose, transferred to a nylon membrane and hybridized with 5′- biotinylated oligonucleotide probes for the rps4, nad3, nad9, atp1, atp4 and atp9 transcripts.