Figure 7.

Transcriptional and translational status of dicistronic nad3-rps12 and rpl5-cob transcripts in P2 and P3 phenotypes of rps10 and wild-type. Coverage of total mRNA reads and ribosomal footprints on the dicistronic (A) nad3-rps12 and (B) rpl5-cob transcript. Reads aligning to the transcript were normalized against the number of all reads in mtRNA-seq and mtRibo-seq libraries and their distribution was visualized using Integrative Genomics Viewer (IGV). (C) Northern hybridization of total mitochondrial RNA using a 5′- biotinylated oligonucleotide probe specific for rps12. Size of dicistronic form of nad3-rps12 transcripts was inferred from transcript 5′ and 3′ ends defined in (7). (D) Northern hybridization of total mitochondrial RNA using a 5′-biotinylated oligonucleotide probe specific for rpl5. Sizes of monocistronic forms of rpl5 and dicistronic form of rpl5-cob were inferred from two 5′ ends and 3′end of rpl5-cob defined in (7) and from cosRNA identified in our analysis in the intercistronic region, putatively coinciding with 3′ end of rpl5. Arrows indicate discussed forms. The membranes were stained with methylene blue and are shown as loading control in the bottom panels.