Fig. 2.

—The distribution and annotation of parallel SNVs in the evolved populations. SNVs that have been observed in >1 replicate population (AF ≥ 0.02) are presented with the number of replicated color coded according to the colorbar at the bottom. The variant annotation is listed on the left according to the following format: Intergenic variants are presented by [ancestral nucleotide][genomic position][evolved nucleotide]. Intragenic variants are presented with the gene symbol [ancestral amino acid][amino acid position][evolved amino acid]. For synonymous SVNs, the evolved amino acid is omitted; STOP codons are marked by *. Ancestral variants are excluded.