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. 2019 Nov 19;48(2):736–747. doi: 10.1093/nar/gkz1103

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Constitutive Akt regulates USP14 IRIF and NHEJ DDR signaling in autophagy-deficient cells. Confocal immunostaining and graphical representation of (A, B) USP14, (C, D) DNA-PKCs-S2056IRIF at the indicated time points in shATG7-inducible LNCaP cells that were treated with dox and preincubated with 10 μM MK2206 for 1 h prior to the 4Gy IR treatment. LNCaP cells were transfected with GFP-PTEN, (E, F) 53BP1 and (G, H) DNA-PKcs S2056 IRIF formation were analyzed in PTEN+ vs. PTEN- cells, following indicated treatments. C4-2 cells were transfected with GFP-PTEN, and (I, J) 53BP1 and (K, L) DNA-PKcs S2056 IRIF formation were analyzed in PTEN+ versus PTEN- cells, following indicated treatments. Nuclei were stained with DAPI. Data shown are the means ± SEM (n = 2); P < 0.05 *, P < 0.01 **.