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. 2019 Nov 14;47(22):11755–11770. doi: 10.1093/nar/gkz992

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Telomere-binding protein aberrations in FBXO47-deficient spermatocytes. (A) Spread spermatocytes from Fbxo47^+/+ and Fbxo47^−/− males were stained with anti-SYCP3 and anti-SUN1 antibodies. Scale bars, 10 μm. (B) Spread spermatocytes from Fbxo47^+/+ and Fbxo47^−/− males were stained with anti-SYCP3 and anti-TRF1 antibodies. Scale bars, 10 μm. (C) Graphs showing the TRF1 intensities per cells (n > 30 cells for each genotype). (D) Spread spermatocytes from Fbxo47^+/+ and Fbxo47^−/− males were stained with anti-SYCP3 and anti-TRF2 antibodies. Scale bars, 10 μm. (E) Graphs showing the TRF2 intensities per cells (n > 30 cells for each genotype). TRF1 signal intensity: Fbxo47^+/+, 8.769 ± 0.351; Fbxo47⁻^/⁻, 9.786 ± 0.6768; TRF2 signal intensity, leptotene: Fbxo47^+/+, 23.873 ± 5.414; Fbxo47⁻^/⁻, 19.660 ± 4.207; Zygotene: Fbxo47^+/+, 38.046 ± 7.281; Fbxo47⁻^/⁻, 18.953 ± 13.110. (F) FBXO47 is expressed in murine germ cells at P10 and P14. TRF2 is destabilized in Fbxo47-deficient germ cells. β-ACTIN served as a loading control.