Figure 1.

Loss of lamin A/C in MEFs results in slower cell proliferation and reduced cell survival. (A) Cell proliferation assays were performed by seeding 25 000 cells per well in 6-well plates, with our without a stressor that causes DNA damage known to be repaired by BER (hydrogen peroxide or MMS). The cells were incubated at standard 37°C/20% oxygen/5% CO_2, and counted each day on a hemocytometer (n = 3, mean ± SD). (B) Cell survival was assessed by the WST-1 assay to estimate the relative number of viable cells at the indicated stressor concentrations. All data points are the mean WST-1 absorbance (as percent of the value from untreated cells) from six wells (in 96-well plates) ± SD. Cells were seeded at 20 000 cells per well, 24 h prior to treatment. Treatment durations for the stressors were as follows: H₂O₂, 4 h; Menadione, 2 h; MMS; 1 h. P values for were determined by two-way ANOVA Sidak’s multiple comparisons test; ***P < 0.0001; **P < 0.005; *P < 0.05