Figure 2.

Loss of lamin A/C in MEFs significantly impairs BER. (A) FPG-comet assay. The MEFs were treated with oxidative stress (100 μM H₂O₂ for 30 min) and then allowed to repair in fresh medium for 8 h. DNA repair efficiency was expressed as percent of FPG-sensitive sites remaining, after 8 h of repair, relative to 5 min of repair, after correction for the FPG-sensitive sites in untreated cells (n = 100 comet tails, mean ± SEM). (B) Immunofluorescence, using the 8-oxoG antibody, was used to monitor the removal of 8-oxoG lesions after 8 h of repair from H₂O₂ treatment (100 μM H₂O₂ for 30 min), relative to 5 min repair, after correction for 8-oxoG accumulation in untreated cells; images from staining with DAPI (to reveal nuclei) and with normal mouse IgG (negative control) are shown in Supplementary Figure S6A. Bar represents 10 μm. Data are expressed as mean fluorescence of 25 cells ± SEM. (C) FPG-comet assays on Lmna^−/− MEFs with forced expression of empty vector (Lmna^−/− EV), lamin A (Lmna^−/− lamin A), or lamin C (Lmna^−/− lamin C); n = 100 comet tails, mean ± SEM. P values were determined by Student’s t-test; **P < 0.005; *P < 0.05.