Table 2B.

Ingenuity top upstream regulators

Upstream regulator	P-value of overlap	Predicted activation state
TP53*	7.05E-35	Inhibited
TGFB1	8.04E-33	Inhibited
TNF	2.44E-26
ERBB2	1.70E-25
HRAS	5.35E-25

IPA to determine top upstream transcriptional regulators associated with Lmna knockout in MEFs. The overlap P-value measures whether there is a statistically significant overlap between the data set genes and the genes that are regulated by an upstream transcriptional regulator. It was calculated using Fisher’s Exact Test, and significance is attributed to P-values < 0.01. If the observed direction of change is mostly consistent with a particular activation state of the transcriptional regulator (‘activated’ or ‘inhibited’), then a prediction is made about that activation state, as shown; TP53, tumor protein p53; TGFB1, transforming growth factor β1; TNF, tumor necrosis factor; ERBB2, erb-b2 receptor tyrosine kinase 2; HRAS, HRas proto-oncogene, GTPase.

*Important tumor suppressor and upstream regulator of several DNA repair pathways, including BER.