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. 2019 Nov 19;48(2):862–878. doi: 10.1093/nar/gkz1092

Figure 4.

Figure 4.

FMRP and N-terminal MOV10 function co-operatively to regulate MREs contained within rG4 in the 3′ UTR of MAZ. (A) IGV screen shot of the region in the 3′ UTR of the MAZ mRNA cloned into the psicheck 2 dual luciferase reporter. ** mutations in the Gs required to form an rG4. #- mutations in the miR-328 MRE site. Top two bars (green): MOV10 CLIP sites. Third bar (blue): FMRP CLIP site. Bottom bar (red): AGO2 CLIP site. (B) Luciferase activity in Mov10 KO N2a cells transfected with the transgenes indicated below, (n = 3), error bars represent SD, **P < 0.001 Student t-test. (C) Luciferase activity in WT N2a cells and WT N2a cells transfected with a construct encoding the KH1 peptide, n = 3, ** P < 0.001, Student t-test. (D) Luciferase activity in WT N2a cells expressing the MAZ 3′ UTR rG4 mutant, (n = 3), error bars represent SD, ** P < 0.001 Student t-test. (E) Luciferase activity in Mov10 KO N2a cells transfected with the MAZ 3′ UTR MRE site mutation (Δ328), the WT MAZ 3′ UTR (tMAZ), the MAZ 3′ UTR rG4 mutant (ΔrG4) and the MAZ 3′ UTR rG4 mutant expressing N-terminal MOV10 and FMRP-ΔRGG, (n = 3), error bars represent SD, *P < 0.05, ** P < 0.001 Student t-test.