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. 2019 Nov 12;47(22):11880–11888. doi: 10.1093/nar/gkz1058

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Unwinding specificity of extended gRNAs and the E values of their unwound state. (A) Schematic and naming convention of extended gRNA used in the unwinding assay. gX20 or ggX20 are gRNA with 5′g and 5′ gg non-hybridizing extensions respectively with canonical lengths of 20 bp in target strand hybridizing region. (B, C) A plot of on-target unwinding activity vs. unwinding promiscuity with gX20 (B) and ggX20 (C). (D) S compared between canonical and extended gRNAs. (E) E values of the unwound state compared between canonical (X20) and extended gRNAs. Error bars represent SD.