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. 2019 Nov 13;47(22):11637–11648. doi: 10.1093/nar/gkz1040

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Reconstitution of the LmaH3 nucleosome. (A) Preparation of histones. Purified histones H2A, H2B, H3.1, H4 and LmaH3 were analyzed by 18% SDS-PAGE with Coomassie Brilliant Blue (CBB) staining. (B) Reconstitution of the H3.1 and LmaH3 nucleosomes. A histone octamer containing H3.1 or LmaH3 (lanes 2 and 3, respectively) was mixed with the palindromic 146 bp satellite DNA fragment (lane 1), and the nucleosomes were reconstituted by the salt dialysis method. The reconstituted nucleosomes were purified using a Prep Cell apparatus, and were analyzed by 0.2× TBE nondenaturing 6% PAGE with ethidium bromide staining. (C) The histone contents of the purified H3.1 and LmaH3 nucleosomes were analyzed by 18% SDS-PAGE with CBB staining (lanes 2 and 3, respectively).