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. 2019 Nov 13;47(22):11637–11648. doi: 10.1093/nar/gkz1040

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The LmaH3 nucleosome maintains an open chromatin conformation. (A) Schematic representation of the nucleosome array reconstituted with 12 repeats of the 177 bp Widom601 DNA. (B and C) Sedimentation velocity analyses of the nucleosome arrays containing the H3.1 (•) or LmaH3 (○) nucleosome, in the absence (B) or presence (C) of 0.6 mM MgCl₂. The enhanced van Holde–Weischet method was used to determine the distribution of the sedimentation coefficients. Three independent experiments were performed and similar outcomes were obtained, using nucleosomal arrays prepared by two independent reconstitutions, shown in Supplementary Figure S4E and F.