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. 2019 Oct 29;48(1):200–211. doi: 10.1093/nar/gkz939

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Non-hydrolysable ATP analog, AMP-PNP, promotes dimer formation. The oligomer distributions were determined from the flexible-oligomer model fits to the SAXS data for Apo, ADP and AMP-PNP forms of DnaA. The samples were dialyzed in buffer prepared in H₂O (A and B) or D₂O (C). (A) Apo-DnaA or DnaA in the presence of 1 μM ADP and 1 μM AMP-PNP. (B and C) Apo-DnaA or DnaA in the presence of 0.5 mM ADP and 0.5 mM AMP-PNP. Error bars reflect the standard deviation from three separate EOM runs.