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H136Q change interferes with nucleotide-induced dimer formation. Similar to WT (Figure 3), SAXS was carried out to determine the percentage of each oligomeric state of DnaA(H136Q) in the absence or presence ADP (0.5 mM) and AMPNP (0.5 mM). The oligomer distributions were determined with the flexible-oligomer model after fits to the SAXS data. Error bars reflect the standard deviation from three separate EOM runs.
