Skip to main content
. Author manuscript; available in PMC: 2020 Apr 10.
Published in final edited form as: Structure. 2020 Feb 5;28(4):393–405.e4. doi: 10.1016/j.str.2020.01.009

Figure 1. Single-Particle Cryo-EM Reconstructions of Mammalian Retromer.

Figure 1.

Four retromer species were resolved between 27 and 6 Å: (A) the retromer heterotrimer; (B) a dimer of trimers; (C and D) retromer chains; and (E) a tetramer of trimers. For each row (A–E), the first column shows two representative 2D class averages for the species, including particle numbers. Scale bars represent 10 nm. The middle two columns show 3D reconstructions (see Figure S2 for contour details) with and without a fitted model. The last two columns show an additional view (rotated by 90°) of 3D reconstructions with and without fitted models. Initial models were generated from partial crystal structures (PDB: 2R17, 5F0J). VPS29 is shown in green, VPS35 in red, and VPS26 in blue or transparent blue (when averaged out in a reconstruction). A schematic of the retromer heterotrimer is shown in (F). See also Figures S1 and S2, Tables S1 and S2.