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. Author manuscript; available in PMC: 2020 Apr 10.
Published in final edited form as: Structure. 2020 Feb 5;28(4):393–405.e4. doi: 10.1016/j.str.2020.01.009

Figure 2. Mammalian Retromer Forms Oligomers in Solution in a Salt-Dependent Manner.

Figure 2.

(A) At 500 mM NaCl (gray trace), the main retromer peak, elutes from a size-exclusion column at a volume consistent with one copy of the VPS26/VPS35/VPS29 heterotrimer (~150 kDa). A small population of retromer elutes in a second peak as a “dimer of trimers” (~300 kDa). At 50 mM NaCl (black trace)the peak profile shifts: the predominant peak is now consistent with four copies of the heterotrimer (600 kDa); a second peak or shoulder is consistent with two copies, and the third is consistent with one copy.

(B) Dynamic light scattering reveals that retromer particles in low salt (50 mM NaCl, gray bars) have approximately double the average radius compared with retromer in high salt (500 mM NaCl, black bars). This is consistent with size-exclusion chromatography with multi-angle laser light scattering data in (A) indicating retromer forms oligomers in solution.