KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CPY	Invitrogen	A-6428; RRID: AB_2536203
Deposited Data
Heterotrimer map	This paper	EMDB- EMD-21136
Dimer map	This paper	EMDB: EMD-21117
Tetramer map	This paper	EMDB: EMD-21101
Chain interface I map	This paper	EMDB: EMD-21116
Chain interface II map	This paper	EMDB: EMD-21118
Flat VPS35/VPS35 sub-structure map	This paper	EMDB: EMD-21135
Curved VPS35/VPS35 sub-structure map	This paper	EMDB: EMD-21119
Heterotrimer model	This paper	PDB: 6VAC
Flat VPS35/VPS35 sub-structure model	This paper	PDB: 6VAB
VPS35 C-terminus/VPS29 structure	(Hierro et al., 2007)	PDB: 2R17
N-VPS35/VPS26/SNX3 structure	(Lucas et al., 2016)	PDB: 5F0J
Experimental Models: Organisms/Strains
S. cerevisiae; SEY6210.1 MATa leu2-3, 112 ura3-52 his3-Δ 200 trp1-Δ901 lys2-801 suc2- Δ9 vps35D::KanMX6	This paper	N/A
Recombinant DNA
GST-VPS35-VPS29 in pGEX4T2 (mouse)	(Collins et al., 2005, 2008)	N/A
H6-VPS26 in pMWKan (mouse)	(Collins et al., 2005, 2008)	N/A
GST-VPS35 (3KE)-VPS29 in pGEX4T2 (mouse)	This paper	N/A
GST-VPS35 (AAA3KE)-VPS29 in pGEX4T2 (mouse)	This paper	N/A
VPS35 in pRS315 (S. cerevisiae)	This paper	N/A
vps35 3KE in pRS315 (S. cerevisiae)	This paper	N/A
vps35 AAA3KE in pRS315 (S. cerevisiae)	This paper	N/A
VPS26 in pRS416 (S. cerevisiae)	This paper	N/A
Software and Algorithms
RELION-2	(Scheres, 2012)	N/A
RELION-3	(Zivanov et al., 2018)	N/A
MotionCor2	(Zheng etal., 2017)	N/A
Gctf	(Zhang, 2016)	N/A
Chimera	(Pettersen et al., 2004)	N/A
PHENIX	(Liebschner et al., 2019)	N/A
3DFSC	(Tan et al., 2017)	N/A
cryoSPARC	(Punjani et al., 2017)	N/A