Maternal CAP treatment improves gut pathology in SHR male offspring. (A) Representative hematoxylin-eosin (H&E) and Masson-trichrome staining images of proximal colon of indicated rat groups. (B-E) Quantitative analysis of Masson-trichrome staining to demonstrate the fibrotic area in the proximal colon. Quantitative analysis of H&E staining to demonstrate the thickness of muscle layer, numbers of goblet cells per 100 epithelial cells and crypt depth in the proximal colon. OCAP, maternal treatment with captopril; SCAP, sustained treatment with captopril. n= 5 rats per group. Data are mean ± SEM. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 in one-way ANOVA followed by Tukey’s multiple comparison.