Figure 6: Microarray analyses reveals minimal gene expression changes in the NP of hTNFα-TG mice.
(A) A heat map showing gene and sample clustering of the genotypes in to two distinct groups with more distance between the hTNFα-TG samples than the WT controls. (B) Significance analysis of microarrays (SAM) with a delta value of 0.74 for an FDR of 5%. (C) Volcano plot showing genes with Log2 (fold change) greater than 1 or less than −1 in orange, genes with Log2 (fold change) greater than 1 or less than −1 and adjusted p value < 0.1 in green, and genes with adjusted p value <0.1 in red. (D) PCR verification of genes identified by microarray analysis with significance determine by t-test. (E) A table showing the DAVID identified significant GO biological terms associated with the gene expression changes identified by the microarray. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.