Histology and mass spectrometry imaging of thermoembolization (negative mode, 9-AA matrix). A, Frozen section H&E of adjacent section as a reference. B, Phosphatidyl Inositol or PI 38:4, 885.521 [M−H]−, (C) oleic acid or OA, 281.2393 [M−H]−, (D) iodide, 126.9014 [M–H], (E) overlay of PI and iodide, (F) overlay of iodide with H&E. Damaged areas correlate with areas with iodide present. G, Taurodeoxycholate or TDC, at m/z 498.2723 [M−H]−, (H) overlay of TDC with PI 38:4, (I) MS of taurocholic acid at m/z 496.2576 [M−H2OH]−, (J) Glyco(cheno)deoxycholate) at m/z 448.2901 [M−H]−, (K) Overlay of Glyco(cheno)deoxycholate) with PI, (L) Combined overlay of PI 38:4, TDC, and glyco(cheno)deoxycholate. Compare localization with D-F depicting distribution of iodide