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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Bone Miner Res. 2019 Dec 27;35(4):714–724. doi: 10.1002/jbmr.3930

Figure 2: PTH (1-34) and abaloparatide similarly improved trabecular bone volume and cortical thickness in mice.

Figure 2:

A) Representative images of right femur showing the changes in trabecular bone in male mice treated with PTH (1-34) or abaloparatide at 4 months of age for 6 weeks. Femurs were prepared for high resolution μCT and images were reconstructed with NRecon software. B - C) A 600 μm cortical volume corresponding to 62 slices of the mid-diaphysis was examined for B) cortical bone mineral density and C) cortical thickness. D - I) A 970 μm volume corresponding to 100 slices of the mid-metaphysis was examined for trabecular bone microarchitecture. D) trabecular bone mineral density (BMD), E) trabecular bone volume (BV/TV), F) trabecular thickness (Tb.Th), G) trabecular number (Tb.N), H) trabecular separation (Tb.Sp) and I) degree of anisotropy. J – M) Femoral thin sections (5 μm) were stained with Masson’s trichrome. Measurements were conducted in the secondary spongiosae for histomorphometric analysis of J) trabecular bone volume (BV/TV), K) thickness of trabeculae (Tb.Th), L) number of trabeculae (Tb.N) and M) space between trabeculae (Tb.Sp). 5-7 mice per group, results are means ± SD. All data were analyzed for normality and equivalence of variance before a 1 way ANOVA followed by a Tukey’s multiple comparison test. The histomorphometric analysis of bone volume did not show equivalence of variance so we used Kruskal-Wallis, a non-parametric test. All exact p values are shown.