Figure 23. CTNNA1 expression at the murine BNB in GDNF transgenic mice following sciatic nerve crush injury.
Digital indirect fluorescent photomicrographs of murine sciatic nerve endoneurial microvessels within 3 hours (Day 0) and 7 days (Day 7) after crush injury in GDNF WT and GDNF CKO mice are shown, with Sham indicating uninjured sciatic nerves. CD31 (green, endothelial cell marker, A, E, I, M and Q) and nuclear marker DAPI (blue, C, G, K, O and S) were performed to identify endoneurial microvessels. Plaque-like linear membrane CTNNA1 expression is partly lost in both GDNF WT and CKO mice immediately after injury, with near complete recovery seen in GDNF WT mice and partial recovery in GDNF CKO mice on Day 7, implying an important role in restoring endoneurial homeostasis after injury. N=2, Scale bar = 2.5 μm.