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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Exp Neurol. 2020 Mar 3;328:113272. doi: 10.1016/j.expneurol.2020.113272

Figure 4. Human BNB Junctional complex.

Figure 4.

Bar histograms demonstrate the effect of exogenous human recombinant GDNF on human BNB tight junctional complex protein expression after 48 hours in vitro. Western blot of confluent pHEndEC membrane protein extracts was performed following SDS-PAGE, using standard protocols with specific validated primary and secondary antibodies. Semi-quantitative expression relative to GAPDH for basal (yellow) and GDNF-treated (green) pHEndECs (A) and GDNF-induced fold-change (B) are shown for each protein, with the ratio of pCTTN/ CTTN shown to indicate whether GDNF modulates pCTTN expression independent of CTTN. * indicates p<0.05, n.s. = not significant. Error bars indicate standard errors of means (N=3). A representative digital western blot image showing GDNF-induced human BNB junctional complex protein expression at 48 hours in vitro is shown, with reduced membrane SEC31A (C)