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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Exp Neurol. 2020 Mar 3;328:113272. doi: 10.1016/j.expneurol.2020.113272

Figure 6. Molecular determinants of the in vitro human BNB.

Figure 6.

ECIS was performed to continuously measure pHEndEC TEER following initial plating for 5 days and 48 hours after serum withdrawal (to induce endothelial cell detachment). Following treatment with GDNF (1 ng/mL) and transfection with validated specific siRNA to inhibit gene transcription or SRC kinase blockade using a Bosutinib, TEER increase was calculated for each well by subtracting the mean lowest TEER during the 1st hour after serum withdrawal from mean maximal resistance at predefined time points, with appropriate negative controls. Histograms demonstrate averages for each time point (A: 24 hours, B: 36 hours, and C: 48 hours) in this single experiment performed in duplicate for each experimental condition.