Figure 20.

Comparison of 193 nm UVPD, HCD, and ETD for middle-down analysis of Lys-C digested Trastuzumab in terms of (a) overlap in identified peptide populations and (b) average peptide mass and sequence coverage obtained by each activation method. (c) Relative E-scores for the 29 overlapping peptides identified by all three activation methods and (d) ion-cleavage maps obtained for an 8.2 kDa CDR-H1 and CDR-H2-containing peptide. The hypervariable regions are shaded in gray. Reproduced with permission from ref 161. Copyright 2017 American Chemical Society.