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. Author manuscript; available in PMC: 2021 Apr 8.
Published in final edited form as: Chem Rev. 2019 Dec 18;120(7):3328–3380. doi: 10.1021/acs.chemrev.9b00440

Figure 27.

Figure 27.

(A) Concept of UVPD with fragment ion protection (FIP) showing resonant excitation to move fragment ions away from the laser beam. (B) Sequence coverage map of UVPD of ubiquitin (12+) using eight 0.25 mJ pulses and expansions of ion profiles and signal-to-noise levels for four large fragment ions without FIP (upper profiles) and with FIP (lower profiles), including y5810+ (m/z 654), a7010+(m/z 787), z639+ (m/z 788), and a7110+ (m/z 798). Results represent the average of three spectra (each consisting of 6 μscans) generated by Thermo Xcalibur Qual Browser v2.2. Reproduced with permission from ref 112. Copyright 2018 American Chemical Society.