Figure 1. Ab-oligo cycIF scheme.

Primary antibodies were conjugated to single stranded oligos (DS = docking strand). After Ab-oligo tissue labeling, the complementary oligo conjugated to a fluorophore (IS = imaging strand) was used for in situ hybridization. After imaging, signal removal was completed using thermal melting, UV light exposure, RE-based oligo cleavage or introduction of thiol for cleavage of a disulfide bond.