Figure 1.

Fluorescence intensity measurements using a BD influx flow cytometer on C. albicans strains expressing different codon optimized versions of fluorescent proteins. Cells were grown overnight on low fluorescent medium, before bringing to an optical density at 600 nm (OD₆₀₀) of 0.2 in fresh Low Fluorescent medium and grown to the exponential phase (4–5 hrs) before measurement. Data shown is corrected for wild type autofluorescence and normalized to the brightest codon variant. A) γmGFP B) mScarlet-I C) mTurquoise2 D) ffDronpa E) YemVenus. Asterisks denote significance level with **** corresponding to p < 0,0001 and ** corresponding to p < 0,01 when comparing with the brightest codon variant.