Figure 3.

Fluorescence intensity measurements using a BD influx flow cytometer on C. albicans strains endogenously expressing (A) CFP or mTurquoise2 attached to Tpk1 and (C) mCherry or mScarlet-I attached to Tub1. Cells were grown overnight on low fluorescent medium and brought to an optical density at 600 nm (OD₆₀₀) of 0.2 in fresh Low Fluorescent medium. Strains were allowed to grow to the exponential phase (4–5 hrs) at 30 °C or 37 °C, before measurement. Data shown is corrected for wild type autofluorescence. Asterisks denote significance level with ***corresponding to p < 0,001. B and D are confocal images taken with a Fluoview 1000 confocal microscope, using the appropriate excitation and emission filters for each fluorescent protein. Shown are representative images of Tpk1 – CFP and Tpk1 – mTurquoise2 (mT2) in panel B and representative images of Tub1 – mCherry and Tub1 – mScarlet-I in panel D.