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. 2020 Apr 9;10:6158. doi: 10.1038/s41598-020-63308-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scatter plot of the fluorescence intensity as measured using FACS versus (A) the folding energy of the structure from the full length (▲) and first 250nt (●) of the mRNA sequence as calculated using RNAfold. Colors denote different fluorescent proteins with orange for YemVenus, blue for mTurquoise2, Light green for ffDronpa and dark green for γmGFP.