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. 2020 Apr 10;5:34. doi: 10.1038/s41392-020-0139-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Sodium chloride (20 mM) or control medium was added to the cultures of ALD-DNA-DCs for 24 h, and then the activation and maturation status of dendritic cells was analyzed by flow cytometry to compare the two groups. b, c Bone marrow-derived dendritic cells from C57BL/6 mice were pretreated with 10 ng/ml LPS and/or 20 mM NaCl for 24 h. These cells were collected for coculture with CFSE-labeled T cells from Balb/c mice for mixed lymphocyte reactions. Flow cytometry analysis of CFSE, which indicates T cell proliferation and indirectly represents the antigen-presenting ability of dendritic cells (b). A CBA cytokine kit was used to measure mediators in the supernatants after DC-T cell coculture for 72 h (c). The results are presented as the mean ± s.e.m. from three independent experiments. ns indicates no significance, **p < 0.005, ***p < 0.0005, and ****p < 0.0001 using paired Student’s t-tests.