Skip to main content
. 2020 Apr 9;10:6127. doi: 10.1038/s41598-020-63335-7

Figure 1.

Figure 1

Vimentin is a miR-144 target in primary cultured cardiac endothelial cells. (A) Transfection. Cultured cardiac endothelial cells were transfected with miRNA and cellular miR-144 levels were determined by stem loop RT-PCR. N = 4, *p < 0.05 and #p < 0.01 compared with SCR which is scrambled control. Dosage (D) 0.5 = 2.5 pmol, D1 = 5 pmol, and D2 = 25 pmol. (B) Incubation. Cultured cardiac endothelial cells were incubated with miRNA and cellular miR-144 levels were determined by stem loop RT-PCR. N = 4, *p < 0.05 and #p < 0.01 compared with scrambled control. D1 = 5 pmol, D2 = 25 pmol, and D3 = 125 pmol. (C) Representative images of two-dimensional gel, n = 2. (D) Quantification of the circled two-dimensional gel spots from panel (C,E) Sequence coverage for mouse vimentin. Mass spectrometry detected peptides aligned with mouse vimentin sequences. The detected peptides are highlighted in red. (F) miR-144 targeting sequence in vimentin gene. (G) Western blot. Cultured cardiac endothelial cells were incubated with miRNA 144 and the culture medium was analyzed by Western blot for vimentin. N = 2. (H) Real time PCR. Cultured cardiac endothelial cells were incubated with miRNA 144 and the cells were harvested for quantification of total RNA. Vimentin mRNA was determined by real time PCR. N = 4. SCR: scrambled miRNA; Vim: vimentin.