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. 2020 Apr 9;10:6127. doi: 10.1038/s41598-020-63335-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Elevation of vimentin in miR-144 KO mice is collocated with atherosclerotic plaques. Plasma vimentin was determined by ELISA (A) and Western blot (B). N = 10 for miR-144 KO mice and WT littermates, *p < 0.05 compared with WT littermates. Vimentin in the aortic root was visualized by immunostaining (C,D) in comparison with that adjacent tissue cuts stained with Oil Red-O (E,F). Each panel of picture is representative of data from 10 mice. The vimentin stains were quantified by Image J and summarized in panel (G) N = 10 for both miR-144 KO mice and WT littermates, ^#p < 0.01 compared with WT littermates. Scale bar in panel C, D, E, and F: 500 µm. Vim: vimentin.