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. 2020 Apr 9;11(4):228. doi: 10.1038/s41419-020-2418-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a ASMase enzymatic activity was measured in AS-M5 CAV1(+) and CAV1(−) cells in control conditions (n = 13). CAV1(−) activity is shown in relation to CAV1(+) cells, that was set at 1. **p < 0.01 by paired t test (two-tailed). b Overview of the most prominent detected ceramide species by LC–MS in CAV1(+) and CAV1(−) EC (n = 3, SEM). *p < 0.05, **p < 0.01, and ****p < 0.001 by two-way ANOVA with post hoc Tukey multiple-comparison test. c, e ASMase enzymatic activity was analyzed after 10-Gy IR. ASMase activity of CAV1(+) (c) and CAV1(−) (e) EC is shown in relation to CAV1(+) unirradiated samples (set as 1) 5, 15, and 30 min after IR treatment (n = 3–4). *p < 0.05, ***p < 0.005, and ****p < 0.001 by one-way ANOVA with post hoc Tukey multiple-comparison test; ns not significant. d, f Timeline of the total ceramide levels generated by CAV1(+) (d) and CAV1(−) (f) EC after IR treatment. Samples were taken 1, 5, 15, and 30 min after 10-Gy irradiation (n = 3, SD). All control (0-Gy) samples were pooled (n = 12). Statistical analysis was done by using Student’s t test with Welch’s correction (*p < 0.05, **p < 0.01). g Timeline of ceramide species C16, C24, C24:1, and total ceramide levels generated by CAV1(+) and CAV1(−) EC after 10-Gy irradiation (n = 3, SD). Samples were taken at indicated time points. Statistical analysis was done by using Student’s t test with Welch’s correction (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001).