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. 2020 Apr 9;10:6164. doi: 10.1038/s41598-020-63213-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of chronic ethanol consumption on neuronal apoptosis in the cerebral cortex following transient focal cerebral ischemia. (A) Representative double staining (10×, scale bar 100 μm) of NeuN and TUNEL in peri-infarct cortex of the temporal lobe at the section 0.23 mm caudal to bregma. (B) Values of TUNEL-positive neuron are means ± SE for 6 mice in each group. (C) Representative double staining (10×, scale bar 100 μm) of NeuN and cleaved caspase-3 in peri-infarct cortex of the parietal lobe at the section 0.23 mm caudal to bregma. (D) Values of cleaved caspase-3-positive neuron are means ± SE for 6 mice in each group. *P < 0.05 vs. Control. Analyzed using one-way ANOVA with Dunnett’s post-hoc.