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. 2020 Apr 9;10:6124. doi: 10.1038/s41598-020-62823-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic diagram of global lipidomic analysis. Total lipids were extracted from 5-FU–sensitive (DLD-1) and –resistant (DLD-1/5-FU) colorectal cancer cells and subjected to MALDI-MS analysis in positive and negative modes in triplicate. MALDI-MS spectra were processed by MALDI-Quant in R package. The transition of sphingolipid and related enzymes (Q1 and Q3) was optimized and quantified using MRM-based LC-QqQ-MS analysis in triplicate. Statistical analysis of quantified lipids was carried out using Metaboanalyst.