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. 2020 Apr 9;10:6124. doi: 10.1038/s41598-020-62823-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MALDI-TOF-MS lipidomic analysis of DLD-1 and DLD-1/5-FU cells. (A) Average chromatogram of DLD-1 (in red) and DLD-1/5-FU (in green) obtained with positive ion mode and (B) negative mode. (C) Principal component analysis (PCA) of the acquired MS spectra from DLD-1 (shown in red) and DLD-1/5-FU (shown in green) in positive and (D) negative modes. Hierarchical clustering of each sample data set showing differentially expressed lipids in (E) positive mode and (F) negative mode. Volcano plots display differentially expressed lipids in (G) positive and (H) negative mode, respectively (fold change > 1.4, p value < 0.05 in positive and fold change > 1.3, p value < 0.05 in negative mode).