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. 2020 Apr 9;10:6126. doi: 10.1038/s41598-020-63228-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the functionalization of P(NIPAm-co-AAc) and IPN microgels with –ene reactive groups. The P(NIPAM-co-AA) and IPN nanogels are is ene-functionalized by allylamine at room temperature in the presence of EDC/NHS couple. Then, allylamine is added to the for the formation of the amide bonds onto the carboxylic acid. The reaction is allowed to proceed for 48 h.