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. 2020 Apr 9;10:6126. doi: 10.1038/s41598-020-63228-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Live/dead assay images for cell viability of cells grown on pristine glass, glass/TMSPMA, PNIPAm, P(NIPAm-co-AAc)-ene and IPN-ene (named IPN). In (a–e) we show fluorescent live/dead assay images representing cell viability. In (f) a statistical analysis of a number of cells dead and the number of cells alive is reported. All data represent the mean values ± standard error, *p < 0.5. Red is for dead cells and green for living cells. The initial seeding density was 20000 cells/cm² and the incubation time 24 h. Red scale bar: 50 µm.