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. 2020 Apr 9;10:6126. doi: 10.1038/s41598-020-63228-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ATR FT-IR spectra of the NIPAm based microgels before and after the amidation reaction with allylamine. Inset: a zoom of the peak at 1725 cm⁻¹, to show the carboxylic acid moiety of AAc. The spectra are normalized with respect to the adsorption band at 1645 cm⁻¹, due to the PNIPAm network.