Figure 1.

Changes in the global transcriptional landscape of chorio-decidua neutrophils compared to blood neutrophils. (A) Chorio-decidua cells were scraped, digested with protease/DNAase and single cell suspensions were used for multiparameter flow cytometry phenotyping. CD45⁺ subsets showed a neutrophil predominance after LPS (n = 19), while Adalimumab treatment (n = 11) partially restored the profile toward controls (n = 18). (B) Principal-component analysis (PCA) of RNA-seq profiling of neutrophils isolated from maternal blood using magnetic beads (open circles: Ctrl n = 3; LPS n = 5; Adalimumab n = 3) and from chorio-decidua by FACS (full circles: Ctrl n = 3; LPS n = 5; Adalimumab n = 4). The expression values were normalized across the entire datasets. (C) Heatmap of genes that are differentially expressed genes (DEGs, adjusted p < 0.05) between blood (n = 3) and chorio-decidua neutrophils (n = 3) in control animals only. (D) Biological processes of differentially expressed genes shown in (C), as determined by Enrichr. Black bars represent upregulated biological processes and open bars represent downregulated biological processes in chorio-decidua neutrophils vs. blood neutrophils from control animals only. (E) Normalized counts of gene expression value of representative genes associated with the biological processes depicted in (D). The lines show normalized counts of gene-expression trend plot from the same control animal (black line= sample #427, red line = sample #432, green line = sample #430; n = 3). ^*p < 0.05 (Unpaired t-test).