Figure 5.

FA attenuated LPS-induced intestinal epithelial barrier dysfunction via activating miR-200c-3p-mediated PTEN/AKT pathway in Caco-2 cells. (A) Cells were transfected with Lv-200c, Lv-200c spong and Lv-NC at an MOI of 15 and incubated at 37°C with 5% CO₂ for 48 h, and then cells were pretreated with FA (100 μM) for 2 h and stimulated with LPS for 24 h. The expression levels of PTEN, PI3K, p-PI3K, AKT and p-AKT were evaluated using western blotting. (B) Caco-2 cells were pretreated with 10 μM LY294002 for 1 h and then treated with 100 μM FA for 2 h. The expression levels of occludin and ZO-1 proteins were evaluated using Western blotting. Data were presented as means ± SD from three independent experiments and differences between means were compared using one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05; **P < 0.01.